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48 hours after ischemia, liver tissue from both ischemic and perfused segments was harvested and oxidized and reduced glutathione was measured with LC-MS and normalized to total hepatic protein

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Major metabolic disorders in MDD include abnormalities in lipid metabolism characterized by diminished reverse cholesterol transport (RCT) and heightened atherogenicity, disruptions in protein metabolism resulting in reduced total serum protein and decreased plasma tryptophan availability to the brain, and nutritional immunity with immune-linked alterations in trace elements such as iron and zinc [5, 6]