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The results were statistically analyzed by the Student's t-test analysis (biological replicates n =3) using GraphPad Prism v.9

1.1342 g of sodium chloride (Fisher Scientific) and 1.53 g of tromethamine (Fisher Scientific) were added

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Specifically, an initial reduction followed by a substantial increase in beta-endorphin levels was observed, a pattern that suggests DSIPs impact on the opioidergic system, potentially playing a role in stress alleviation or adaptation processes

Older people usually produce less stomach acid and therefore less intrinsic factor, increasing their risk for deficiency