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For Safranin O/Fast Green staining, slides were stained with hematoxylin (Mayer, Sigma) for 5 min, 0.005% Fast Green (Thermo Fisher Scientific, Pittsburgh, PA, United States) for 1 min, rinsed with 1% acetic acid, and stained with 0.1% Safranin O (Millipore, Billerica, MA, United States) for 20 min

Dendrite length was determined using the National Institutes of Health's Image J 1.41o program (NIH, Bethesda, MD) and the neurite tracing program Neuron J (Meijering et al., 2004) Spines were manually counted

Introduction# CagriSema and retatrutide represent two of the most anticipated next-generation obesity treatments, each taking a fundamentally different multi-target approach to weight management

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