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Notably, incubation of AX with L-cysteine (with the nonprotected-amino group) did not afford DKP, but mainly, the product corresponding to the aminolysis of the -lactam ring, which remained conjugated to the amino group of cysteine through an amide bond ( Figure S8 )

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Overall, these differences suggest the contributions of FGF21s diverse metabolic effects vary across species

Key Challenges in Glutathione Conjugation Projects Unclear Site Selectivity Glutathione contains a highly reactive cysteine thiol together with terminal amino and carboxyl functions, so route selection matters