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Subsequently, a NaBH 4 solution (0.1 mg/mL, 50 mL) was slowly added to the solution, and stirring was maintained for 20 min at room temperature

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Same vial, different bac water volume = different concentration = different syringe math

While highly informative, these approaches frequently fail to capture the influence of systemic or extracellular signals such as estrogen [6] or glucocorticoids [7]