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Intracellular sulphhydryl (thiol) groups are also vital for the catalytic function of many enzymes

The pyruvate decarboxylase reaction mechanism Phase 1: Decarboxylation of pyruvate The \(ThDP\)-stabilized carbanion then acts as a nucleophile, cleaving the disulfide bridge of lipoamide (step 3 below)

Lancet 366 , 985990 (2005)

The enzyme does not require added PLP but is inhibited by carbonyl reagents suggesting the presence of a carbonyl-containing cofactor

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Even though the Mendelian randomisation is a powerful analysis, further animal experiments are required to unravel the molecular mechanisms and validate these findings