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Measurement of intracellular ROS (DCFDA) The level of intracellular ROS was detected using cell permeable fluorescent probe 2, 7-dichlorofluorescin-diacetate (DCFDA)

[DOI] [PubMed] [Google Scholar] Danbolt NC

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therefore, the over-expression of IGF-I has been associated with its tumorigenic activity, protecting the cancer cells from apoptosis (Prrizas et al., 1997)