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When this gene cluster is activated, these enzymes mediate the production of gliotoxin from serine and phenylalanine residues

This suggests that it is likely that acetylphenylhydrazine undergoes a cyclic redox reaction that results in the production of hydrogen peroxide, which in turn oxidises glutathione

& Thiele, I

Hopkins published the complete structure in 1935, establishing that the thiol (-SH) group on cysteine was the reactive site responsible for the molecule's reducing properties

The samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose (NC) membranes (GE Healthcare)

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