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iTRAQ samples were tryptically digested by using a FASP Protein Digestion Kit (Protein Discovery, San Diego, CA) according to the manufacturers instructions with slight modifications (Supplemental Data), samples were desalted by C18 solid-phase extraction (SPE) before isobaric labeling (SUPELCO)

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He added that B12 deficiency "can be undiagnosed or underdiagnosed since symptoms can be nonspecific," like cognitive slowing, fatigue, tongue swelling, arm or leg tingling, and difficulty walking