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Point mutations and NNK site saturation libraries of PtGSTF8 were constructed by overlap extension PCR as previously reported 49 using primers T7 fw, T7 term and: PtGSTF8 (V12X): V12C13_rv and V12X_fw, PtGSTF8 (C13X): V12C13_rv and C13X_fw, PtGSTF8 (C13S): V12C13_rv and C13S_fw, PtGSTF8 (N108X): N108_rv, N108X_fw, PtGSTF8 (F112X): F112_rv, F112X_fw (Supplementary Table 4) using pANT35 as template

Body cells and tissues are threatened continuously by damage caused by toxic free radicals and reactive oxygen species

Animal experiment in accordance with EU guidelines under ethical approval (Maastricht University, AVD1070020185464)

Gently squeeze the pipettes bulb before inserting the pipette tip into the bacteriostatic water

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The endothelium can also regulate inflammation and vascular smooth muscle cell proliferation [49]