Typically, patients are either given a strong topical numbing cream or they are injected with lidocaine to numb the area that will be treated
BSO + Recovery: Cells treated with BSO for 20 hours, washed 2X with PBS and covered with fresh media without BSO

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It is also known that oral contraceptives may lower pyridoxine levels in some individuals, which could be considered a drug-nutrient interaction of clinical relevance
Wang X, Zhou Q, Yu Z, Wu X, Chen X, Li J, et al
Shoulder & Upper Extremity Plano Orthopedic & Sports Medicine Center Plano, TX