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The placement of waters was confined to AMY 1 R:Cagri and CTR:Cagri ( N = 1) within the deep binding pocket in order to avoid over interpretation of the cryo-EM maps
Collected samples centrifuged at 3000 g for 20 min
After OGD treatment, the number of Ki-67-positive endothelial cells significantly decreased, while the intracellular expression levels of senescence markers P21, P53, and the DNA damage indicator -H2AX were markedly elevated (Figure 3B)
Besides PEG, there are other reagents used in improving the pharmacokinetics of peptides
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These investigations focus on laboratory-based assessment of peptide structure, molecular interaction behavior, and stability characteristics under experimental conditions
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