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Then antigen retrieval was performed with 1X Target Retrieval Solution, pH 6.0 (Dako) at 125 C for 4 min in a Retriever 2100 (Aptum Bio), followed with blocking using 10% goat serum in PBS for 60 min at RT

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The principal component scores were then calculated for each treatment group and normalized to the 01 range using the affiliation function

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