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This simple act removes a huge vector for contamination

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Two-step assay with fixation prior to chase step: - DBCOcl incubation, formaldehyde fixation, and azido-tagged dye DBCO-labeled cells prepared as described earlier were washed 3X with PBS and resuspended in 4% formaldehyde in PBS and incubated statically at room temperature on the benchtop for 30 min

NAD+ infusions run two to four hours because a faster rate causes discomfort

This effect is believed to be linked to reduced oxidative stress and improved mitochondrial efficiency

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