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Mosquito hemocytehaemocyte-mediated immune responses

Nowinski, R

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For cell samples, cells were lysed with lysis buffer (50 mM Tris-HCl [pH 8.0], 1% Triton X-100, 0.5% Nonidet P-40, 10 mM dithiothreitol, 1% protease inhibitor cocktail, 150 mM NaCl, and 5 mM EDTA) on ice for 30 min, followed by centrifugation (12,000 g , 20 min, and 4 C)

Most of these metals/metalloids accumulate in organs upon chronic exposure [130] and their biological effects may contribute to the shortening of the organs lifespan, mainly the kidneys, and ultimately lead to their early failure [103]

Nature 468 (7327), 11151118