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Foot flexion contracture in the rats were evaluated and graded on a scale of 0-4 with 0 being no contracture

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Storage duration critically impacts effectiveness - reconstituted GHK-Cu lasts only 21-28 days refrigerated due to copper oxidation concerns (shorter than most peptides), requiring strategic reconstitution of amounts usable within this window rather than preparing entire vials at once

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Mixing alcohol with these drugs can affect blood clotting, raise injury risk and make some medications harder to manage