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The protein free supernatant was collected and mixed immediately with 0.5 mL of NADPH (4 mg of reduced form was dissolved in 100 mL of 0.5% NaHCO 3 ), 0.5 mL of glutathione reductase (6 units/mL in 0.1 M phosphate buffer, pH 7.0) and 1 mL of 0.6 M DTNB (prepared in 0.2 M phosphate buffer of pH 8)

We, therefore, speculate that these signaling differences between DA and NE may provide islet cells with an additional mechanism for finer temporal control of hormone release that may be especially critical for differential metabolic adaptations to states of acute and chronic stress when concentrations of circulating catecholamines are altered