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Aging 2 , 914923 (2010)

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Effects of flavonoid phytochemicals on cortisol production and on activities of steroidogenic enzymes in human adrenocortical H295R cells

Cell viability was measured by assaying mitochondrial activity using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) transformation, as previously described ( Measurement of Intracellular Ca 2+ Concentration Intracellular calcium concentration ([Ca 2+ ] i ) was measured using the fluorescent indicator fura-2

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