The mechanism is well-characterized and scientifically sound NNMT overexpression in obese adipose tissue is well-documented, and its inhibition does restore metabolic function in cell culture and animal models
The lower end showed measurable effects on fat metabolism but slower results
This concentration allows for precise dosing across the typical 300-500mcg range with standard insulin syringes
(Nature, 2014) used an antisense oligonucleotide to knock down NNMT in mice and reported reduced fat-mass gain, better glucose tolerance, and lower liver triglycerides the founding paper for the whole NNMT-inhibitor field
Guide for the Care and Use of Laboratory Animals , 8th ed, 220 (National Academies Press, 2011)
Cyanocobalamin (b12) injection 2,000 mcg / mL Multiple dose for intravenous, intramuscular or subcutaneous use