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Non-V600 BRAF Mutations Define a Clinically Distinct Molecular Subtype of Metastatic Colorectal Cancer

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After electrophoresis, blotted proteins to polyvinylideneflouride (PVDF) membranes were blocked for 1 h in a Trisbuffered saline (TBS-T) blocking solution containing 5% (w/v) non-fat milk and 0.05% Tween-20

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