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DiNicolantonio et al

The shRNA sequence (TRCN0000322995) targeting ATG10 was designed according to MISSION shRNA Plasmid DNA (MERCK) and synthesized and joined the FastDigest BshTI/FastDigest EcoRI linearized (Thermo Fisher) pLKO.1-GST DAF vector with T4 DNA ligase (EL0011, Thermo Fisher)

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tRNA methyltransferase homologous A gene mutation causes an autosomal-recessive genetic disorder commonly characterized by microcephaly, diabetes mellitus, and epilepsy

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Precautions and Contraindications People with certain health conditions, such as asthma or kidney problems, should use glutathione supplements with caution