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The glutathione assay method utilizes an adaptation of Ellmans Reagent (DTNB), where 5,5-dithiobis (2-nitrobenzoic acid) (DTNB) reacts with GSH to form the compound TNB (2-nitro-5-thiobenzoic acid), which is then quantified using HPLC analysis

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Mechanism of Action Acetyl Group Protection : The attached acetyl group acts as a molecular shield, preventing digestive enzymes from breaking down glutathione prematurely in the stomach and intestines, which allows the intact molecule to reach target tissues like the liver, lungs, and brain

RCT, Randomized Controlled Trial

This mechanism controls energy production, detoxification, and emotional balance