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S-adenosylhomocysteine hydrolase (SAHH) then hydrolyzes SAH into homocysteine, a metabolite that must be efficiently cleared to prevent toxicity

Figure 6 3.8 CB-839 did not improve cytotoxicity in combination with venetoclax, AZD-5991, and ibrutinib in primary CLL lymphocytes We then tested these drug combinations in the primary CLL cells, both in the native condition ( Figure 7A ) and in media supplemented with cytokines (CD40 ligand and interleukin-4) to mimic the microenvironment and maintain viability ( Figure 7B )

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To overexpress HNF4 and SAA genes, pcDNA3 vector (ThermoFisher Scientific) and pcDNA3 cDNA ORF clones of HNF4 (OHu25290D), MAT1A (OHu18604D), BHMT (OHu17861), CBS (OHu26151), and CDO1 (OHu17128) from Genscript USA were transiently transfected into the indicated cells with Lipofectamine 3000 (ThermoFisher Scientific)