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For each condition 1 mL supernatant was collected from the 150 cm 2 flask, centrifuged at 10,000 g and 4 C for 1 min, 500 L were transferred into a 1.5 mL collection tube, snap-frozen in liquid nitrogen, and stored at 80 C until analysis

NRF2 and tissue homeostasis In normal cells exposed to oxidative insults, these pathways work in concert to produce building blocks for the replacement and repair of damaged components

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Zhou Y, Li J, Gordon SC, Trudeau S, Rupp LB, Boscarino JA, Daida YG, Schmidt MA, Lu M

Some report improved sleep quality or subtle increases in energy, though these could reflect placebo effects