The exception is other copper-containing peptides, where cumulative copper exposure becomes a consideration
Therefore, a multiple standard addition approach was employed for method validation, enabling accurate quantification despite significant matrix effects without requiring isotope-labeled IS
doi: 10.3390/nu13072421 111 MosherAATsoulisMWLimJTanCAgarwalSKLeylandNAet al
Lyophilization The SLN dispersion was freeze-dried using a lyophilizer (Ishin Biobase, South Korea)
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Bhosale SD, Moulder R, Kouvonen P, Lahesmaa R, Goodlett DR (2017) Mass spectrometry-based serum proteomics for biomarker discovery and validation