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As indicated in Figure S4A , in the region of the graph above the lines, Niclosamide is in solution, predominantly as Nic -ve the negatively charged salt (up to its solubility limit, measured later to be 640M at pH 9.5)

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Compared with normal LETO rats, islet cells in OLETF rats were mostly destroyed, and therefore few beta cells were detected by immunostaining with the anti-insulin antibody

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