SNIP1 mutations in the FHA domain affect cell viability and pre-mRNA splicing To determine whether mutations in the SNIP1 FHA domain affect incorporation of SNIP1 into the activated spliceosome, we expressed flag-tagged SNIP1 (F-SNIP1) WT, single mutants K265G, K301G, R365G, E366G, and the double mutant K265G/K301G in cells and compared their association with components of the activated spliceosome (Fig
Specifically, heat stress led to a marked increase in AsA levels by 57.3%, while DHA content decreased by 11.9%, resulting in a significant enhancement of the AsA/DHA ratio by 78.1% compared to the control conditions
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Frequently Asked Questions / Reference G257: GSSG/GSH Quantification Kit Revised Jul., 29, 2024
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