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Following complete detachment of cells from the wells, 2ml of media was added to neutralize the trypsin and an aliquot was used to determine cell number using a hemocytometer

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Spray Calculation Total Volume: 5ml Spray Output: 0.1 mL/spray (standard metered pump) Total Sprays: 5mL 0.1 mL/spray = 50 sprays Dosage per Spray Total DSIP: 10 mg = 10,000 mcg Total Volume: 5 mL Concentration: 10,000 mcg 5mL = 2,000 mcg/mL Dosage per Spray: 0.1 mL 2,000 mcg/mL = 200 mcg DSIP per spray

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, 94 , 132-137, doi: Njlsson, R., Carlsson, K., Winkler, A., Larsson, A., and Norgren, S