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GSH content can be calculated indirectly by measuring the OD value at 450 nm

J Dermatol 33(10):662669 Nishinaka Y, Masutani H, Nakamura H, Yodoi J (2001) Regulatory roles of thioredoxin in oxidative stress-induced cellular responses

Thymosin Beta are peptide is created from Thymosin beta 4

Subsequently, the protein was eluted with elution buffer and the fractions containing the protein were transferred to a Pierce protein concentrator PES (10-kDa molecular weight cutoff, 520 ml