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Perumpail BJ, Khan MA, Yoo ER, Cholankeril G, Kim D, Ahmed A (2017) Clinical epidemiology and disease burden of nonalcoholic fatty liver disease

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10.1016/S00029343(01)00849-X 34

Isolation of adipocyte-rich fraction For adipocyte fractionation, freshly harvested tissue was finely minced with scissors and digested using Collagenase Type II (1 mg/ml, Thermo Fisher Scientific, Waltham, MA, United States) dissolved in PBS (Thermo Fisher Scientific, Waltham, MA, United States), supplemented with 10 mM CaCl2 (Sigma-Aldrich, St