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The resin was washed once with His lysis buffer and three times with His 6 wash buffer (50 mM Tris-HCl pH 7.5, 300 mM NaCl, 20 mM imidazole, 5% glycerol, 2.5 mM ME)

A Selective Glutathione Probe based on AIE Fluorogen and its Application in Enzymatic Activity Assay

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It's a shorter fragment of the BPC 157 sequence, consisting of only 11 amino acids

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