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Mutated GST sequences were subcloned into pET-15b, pET-24d, pGEX 4T-1, and pCMV-2B expression vectors, and the mutated constructs were confirmed by DNA sequencing

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In 1957, the famous biochemist Albert Lehninger studied vitamin C biosynthesis in animals, and realized that, unlike many species, such as cats and dogs, which can biosynthesize their own vitamin C supply, humans are unable to do so

Namely, redox-active metals, like Fe 2+ , react with peroxides generating highly active hydroxyl radicals (R-HO ) that further propagate the peroxidation reaction (43)