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With advancing age, microglia also become primed, responding to inflammatory stimuli with exaggerated cytokine and free radical release [278]

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Then cDNA was synthesized using High-Capacity cDNA Reverse Transcription Kits (4374967, Applied Biosystems) with the accompanied random primers, as described previously ( The mRNA expression levels of given genes in the transfected cells with or without cell sorting enrichment were determined by qPCR using Taq388 mix (Supplementary Table S3

complemented by genetically encoded sensors for compartment-specific, real-time tracking and CRISPRCas systems for GSH-dependent genomic reporting, these integrated platforms collectively facilitate real-time monitoring, precise biomarker detection, and the development of redox-targeted therapies

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