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Immunofluorescence Imaging Organoids were cultured with 15 l Matrigel (BD, 356231) on chamber slides (Falcon, 354108) covered with 200 l IntestiCult Growth Medium supplemented with 100U/ml penicillin and 100 g/ml streptomycin (Biochrome, 0257 F)

The primary activation pathway is initiated when MST1/2 cofactor forms a complex with SAV and activates LATS1/LATS2 by phosphorylation (Hao et al., 2008)

A concerted effort to standardize protocols, optimize delivery strategies, and conduct well-designed randomized trials is essential to establish MSC-based therapy as a clinically viable solution for oncofertility preservation

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10.1016/j.freeradbiomed.2020.10.002 Free Radic Biol Med