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Antibodies against HIV were tested in all groups to confirm that all participants were negative for HIV through a pre-screening that included a comprehensive metabolic panel, HbA1c levels, hepatitis B surface antigen, and pregnancy test (if applicable)

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DES(1-3) IGF-1 retains native IGF-1's 67-amino acid size and has a plasma half-life of only 20-30 minutes, similar to native IGF-1, whereas IGF-1 LR3's additional bulk and IGFBP evasion extend its half-life to 20-30 hours

This step in the isolation process helps ensure a high signal-to-noise ratio