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The plate was stirred and stored in fridge at 4 C during the experiment

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Cell culture Human aortic SMC (HAoSMC) were purchased from ATCC (PCS-100-012, ATCC) and propagated in Vascular Cell Basal Medium (PCS-100-030, ATCC) supplemented with Vascular Smooth Muscle Cell Growth Kit (PCS-100-042, ATCC) as per the manufacturer's protocol in a standard cell culture incubator at 5% CO 2 , 37 C

IK and SS were responsible for the methodology