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Add the clear sample containing target GST-tagged protein onto the equilibrated gravity column, keep the sample in column for at least 2 min, ensure that the sample fully binds with the resin, and collect the flowthrough with a flow-rate of 0.5 - 1 mL per minute, then add the flowthrough onto the column again, repeat the previous operation, this can increase the binding efficiency

Avoid using it with retinol or other strong acids, and make sure you apply sunscreen

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pH and stability optimization Retatrutide peptide stability is influenced by the pH of the reconstituted solution