Separated proteins were transferred onto Immobilon-P Transfer membranes (MilliporeSigma, IPVH00010), blocked for 1 h using 5% BSA in TBS0.5% Tween-20 and stained overnight with primary rabbit-anti-GCLC (Sigma-Aldrich, HPA036359) or affinity-purified rabbit-anti-GGT1 (GGT129, as previously described 63 ) 1:1,000 in BSA 2.5% overnight at 4 C
Glutathione exists in two forms as free glutathione: the reduced thiol form, which is abbreviated GSH the oxidized disulfide form, which is abbreviated GSSG A thiol is a sulfur compound that contains a sulfhydryl group (-SH), which is a sulfur atom attached to a hydrogen atom
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Its genome is a circular, partially double-stranded DNA, which encodes four overlapping open reading frames: C (core, HbcAg), X (HBx), P (DNA polymerase) and S (surface antigen, HbsAg)
An aliquot was taken and evaporated in an oven at 50C to get the weight of the total lipid
Usman SS, Dahiru M, Abdullahi B et al
