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Cell counting kit (CCK)-8 analysis KGN cells (2 103 cells/well, 100 L/well) were plated in 96-well plates and cultured overnight, followed by continued culture for 72 h for CCK-8 assay

Suppository delivery of these nutrients is comparable to intravenous drip

Inject in the same abdominal area, rotating slightly each session

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Compared with EVs derived from cultured mammalian cells, stem cells, or immune cells, MEVs offer unique advantages in terms of safety, scalability, robustness, and translational feasibility

What does a correctly reconstituted solution look like