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Mitochondrial dysfunction in diabetic neuropathy: a series of unfortunate metabolic events

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Hence, multiple systems including MDHAR, DHAR, glutathione, and ferredoxin contribute to the generation of reduced ascorbate

On the day of assay, the mannitol and sucrose (MAS) buffer (1) was prepared by diluting sterile-filtered MAS 3 in distilled water, resulting in a solution with the following final concentrations: 220 mM mannitol, 70 mM sucrose, 10 mM KH 2 PO 4 , 5 mM MgCl 2 , 2 mM HEPES, 1 mM EGTA and 0.2% w/v fatty-acid free BSA (3117057001) (pH 7.2), and warmed to 37 C

The temperature cycling from cold to warm to cold again creates stress on the peptide structure