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TBIs are categorized based on severity as mild, moderate, and severe based on clinical parameters such as loss of consciousness, amnesia, neurological symptoms, and the findings from structural brain imaging, such as CT or MRI scans (Figure 1 shows the pathophysiology and SICs associated with TBI

After TMB substrate solution is added, only those wells that contain Rat IL10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color

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While B12 is generally safe, caution is needed in specific clinical situations: Lebers Disease (Hereditary Optic Neuropathy): B12 injections are generally contraindicated or should be used with extreme caution, as they may potentially aggravate optic atrophy in this rare condition

References: [1]afak, Martin

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