E., Bonnema, S
Phase 1: loading (weeks 1 to 8) The loading phase establishes tissue levels and initiates regenerative processes
High Oxidative Stress: Smokers, heavy alcohol users, polluted environments, chronic stress
Glutathione supplements can be taken orally, but they have to travel through the digestive system first, which weakens their potency
NS optional) Vita Blend B1 300mg, B2 6mg, B3 300mg, B5 6mg, B6 6mg Amino Blend Proline 200mg, Arginine 400mg, Lysine 200mg, Citrulline 200mg Magnesium Chloride 600mg Zinc Sulfate 10mg Ascorbic Acid 2000mg Alpha Lipoic Acid (ALA) 100mg (pre-infusion push) Glutathione 800mg (post-infusion push) NAD+ 300mg (secondary NS or LR 1000ml bag
After blocking in 5% skimmed milk in PBS containing 0.05% Tween 20, membranes were probed with anti-human Lyn monoclonal mouse antibody (Santa Cruz) diluted 1:1000, or anti-human IL-1 sRII mouse antibody, Clone 8.5 32 followed by detection with anti-mouse-IgG-horseradish peroxidase conjugate diluted at 1:5000 in blocking buffer (Stratagene)