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Dissected cochlear epithelia are transferred to permeable membrane inserts (Millicell organotypic culture plate inserts, Millipore) which are placed into 1 mL of culture media (Dulbecco's modified Eagle's medium (high glucose, Glutamax, 25 mM HEPES) with 10% fetal bovine serum, 1% N2 supplement and 10 units/ml penicillin) in a 6-well plate

[4] [6] Ascorbic Acid (Vitamin C) Injection is contraindicated in individuals with a known hypersensitivity to ascorbic acid or any component of the formulation

CONCERN #7: BRAIN FOG AND COGNITIVE DECLINE Description: That frustrating feeling of mental cloudiness, poor memory, and an inability to focus

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A saline-mixed vial must be used within 24 hours

Soeters et al., 2018)