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Small interfering RNAs (siRNAs) targeting AMPK were transfected into hepatocytes using Lipofectamine Gold, following standard protocols

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Schedule: One subcutaneous injection a week across an 816 week course, with the dose raised in phases

Clear documentation reflects controlled production standards

16 The needle is inserted along the inferior medial border of the scapula and directed parallel to the plane of the undersurface of the scapula, not toward the chest wall (Figure 4)

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