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The level of intracellular glutathione is a key regulator for the induction of stress-activated signal transduction pathways including jun N-terminal protein kinases and p38 kinase by alkylating agents

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The samples were subsequently washed with 1 ml of 1% BSA and 0.5% Tween-20 in PBS, centrifuged and the pellets were resuspended in 1 ml of PI solution (20 g ml 1 propidium iodide (Sigma-Aldrich, P4170), 0.5 mg ml 1 RNase A (Sigma-Aldrich, R5503) and 0.1% Triton X-100 in PBS)

Four other studies reported no statistically significant differences in glutathione levels in the posterior medial frontal cortex [23], mPFC [24] or anterior cingulate cortex (ACC) [25, 26]

Hydration matters

doi: 10.1519/JSC.0000000000001431