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Lysed cells (20 l) were transferred to a new 96-well plate and supplemented with 80 l water

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Oocytes that failed to develop into the GVBD stage after 2 h were discarded, and the remaining GVBD stage oocytes were then continuously incubated to assess PBE development, indicated by the production of first polar body, in 14 h, 16 h and 20 h afterward

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