Med 10 , 1947 (2021)
Flow cytometric analysis The separated leukocytes were stained with fluorescent-conjugated antibodies purchased from BioLegend, BD Biosciences (Franklin Lakes, NJ, USA), Thermo Fisher Scientific, and Tonbo Biomedicine as follows: anti-CD3, anti-CD4, anti-CD8, anti-CD11b, anti-CD11c, anti-CD45, anti-CD45R(B220), anti-CD95, anti-CD117(c-kit), anti-CXCR5, anti-FcRI, anti-GL-7, anti-IgE, anti-Ly6C, anti-Ly6G, anti-PD-1, and anti-Siglec-F (Supplementary Table 1)
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Abbreviations BBD: Box-Behnken design BSA: Bovine serum albumin DTNB: (5,5-dithiobis-(2-nitrobenzoic acid) GPx: Glutathione peroxidase GSH: Reduced glutathione GSSG: Oxidized glutathione LOD: Limits of detection LOQ: Limits of quantitation RSM: Response surface modeling TBuOOH: Tert-Butyl hydroperoxide TNB: Nitro-2-thiobenzoate anion WSAVA: World Small Animal Veterinary Associations References Ademi M (2023) The role of ionized water and its impact on the activity of glutathione peroxidase on rats blood serum during hyperthermic stress