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(4) Centrifuge for 10 min (12000 rpm, 4C), aspirate 150 L of the supernatant with a syringe, filter it using a 0.22 m organic phase pinhole filter, transfer it to an LC injection vial and store it at -80C until LC-MS analysis

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Mistake #3: Shaking the Vial

In the early 1990s, researchers began focusing on the role the pineal gland plays in the aging process, specifically, its ability to modulate functions of the neuroendocrine and immune systems, which have been shown to decease with age

However nonalcoholic fatty liver disease (NAFLD) is increasingly recognized as a cause of isolated abnormalities in serum transaminase concentrations

PMC 5787624