It also helps protect cells from further harm by reducing inflammation and defending against microbes
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If, however, the volume of the red methyl-B12 shot is small, the urine will not show red or pink even though the methyl-B12 is filtered through the kidney into the urine within the same 30-45 minutes after being injected

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The extracts were centrifuged at 10,000 rpm for 10 min at 4 C, and the supernatant was collected and used immediately for the assay at a dilution of 1:10 in PBS/EDTA