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The amplified E2E1 and Ubc9 genes were cloned into the pET28a-SUMO expression vector, and the K136R and K14R mutations were introduced into the E2E1 and Ubc9 genes, respectively, by PCR

In Block 2, we entered an additional binary dummy variable, Residual Schizophrenia, coded 1 for a patient meeting criteria for residual schizophrenia, and 0 otherwise

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To determine whether increases in intracellular GSH directly enhance expression of T2-signature proteins in HAECs, we lowered intracellular GSH levels by inhibiting SLC7A11 using the chemical inhibitor erastin (16)

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